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 5c–d). CX-5461-mediated S33 phosphorylation of RPA was impartial of your mobile cycle stage and was not limited to the nucleoli in HR-deficient cells. Consequently, the development of ssDNA buildings in CX-5461 taken care of cells may result in replication fork stalling and ATR activation with HRD exacerbating CX-5461-mediated replication press